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Smithsonian National Zoo and Conservation Biology Institute

1 ARTICLES PUBLISHED IN JoVE

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Biology

A Semi-Automated Workflow for the Cryopreservation of Coral Sperm to Support Biobanking and Aquaculture
Jonathan Daly 1,2, Rebecca Hobbs 1, Nikolas Zuchowicz 3, Mary Hagedorn 4,5, Justine K. O’Brien 1,2
1Taronga Institute of Science and Learning, Taronga Conservation Society Australia, 2School of Biological, Earth and Environmental Sciences, University of New South Wales, 3Department of Mechanical Engineering, University of Minnesota, 4Hawaii Institute of Marine Biology, University of Hawaii, 5Smithsonian National Zoo and Conservation Biology Institute

This protocol describes a semi-automated pathway to improve the efficiency and capacity of processing and cryopreservation of sperm from threatened coral species, aiming to secure genetic diversity and support reef restoration efforts.

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